Environmental & Occupational Health Sciences Institute (EOHSI) / Rutgers Cancer Institute of New Jersey

News

Oct. 2012

The new Gallios Flow Cytometer with 10 colors, 3 lasers is available.

 

Jan. 2010:

Core Facility is please to introduce the instrument : BD FACSArray Bioanalyzer.

 

Jan. 2009:

The new state-of-the-art instrument, Beckman Coulter MoFlo XDP Cell Sorter, is avaliable for BL2 cell sorting.

 

 

 

cell cycle-2

 

 

histogram

 

 

 

Dot_plot

 

 

 

 

 

 

 

 

Rutgers

EOHSI

CEED_NIEHS

Beckman Coulter FC500 Analyzer

BrdU_Cell cycle
 

The Cytomics FC500 is a user-friendly analytical instrument with two laser excitation wavelengths, 488nm (air-cooloed Argon ion laser, 20mW output) and 633nm (air-cooled Helium-Neon ion laser, 20mW output), that can measure up to 5 colors simultaneously depending on which laser is turned on.

A continuous pressure is applied to the sample tube based on user selected flow rates. Three flow rates are available: low (around 15 Ál/min), medium (around 30 Ál/min), high (around 60 Ál/min).

Particle size range to analyze is 0.5 µm to 40 µm diameter.

This cytometer has the additional capability of 'walk-away' operation. It has a carousel that may be loaded with up to 32 tubes each to be run automatically.

Two FC500 analyzers are available currently in EOHSI building. Facility personnel provides help with the instrument acquisition, data analysis, and consultation for experiment design. Training is also available to enable investigators, their staffs, and graduate students to run the analyzer by themselves. Please contact us.

Excitation
Detectors
Emission
Fluorochromes
488nm
FL1
525nm
FITC, AF488, GFP, YFP, CFSE, Rhodamine123, Vybrant Green
488nm
FL2
575nm
PE, DsRed, Vybrant orange
488nm
FL3
620nm

PE-Texas Red(ECD), PI

488nm

633nm

FL4
675nm

PE-Cy5, PerCP, 7AAD

APC, AF 633, AF647

633nm
FL5
755nm
PE-Cy7, APC-Cy7, APC-AF750

 

Location:  EOHSI Building, Room 347 ModFit

Available software for analyzing data:

  • Kaluza Analysis Software is available.
  • You can analyze your data on your computer using Kaluza analysis software. First, download the one-month free demo Kaluza software on your computer by clicking here. Second, ask us to borrow a dongle for your computer to use the Kaluza analysis software.

    To download of Kaluza Instruction : click here

  • CXP Anlaysis, Beckman Coulter
  • ModFit LT, Verity Software House, Inc.

 

 

Tips for preparation of samples:

  • Cell concentration in each tube: about 1 million cells/ml
  • Sample volume in each tube: 0.5 - 1 ml (minimum volume is 0.5 ml)
  • Sample tubes must be polypropylene (12x75 mm round bottom polypropylene tube- BD Falcon Cat# 352002 for non sterile, or Cat# 352063 for sterile).
  • All samples should be filtered with 40 Ám nylon cell strainers (Falcon # 352340) prior to running to avoid clogging the machines.
  • If you have samples stained with more than one fluorochrome per sample, we recommend you  bring single color stained positive control samples (each fluorochrome individually) and unstained or negative control (isotype) cells for proper compensation.
  • We recommend that samples are kept on ice or chilled until ready to run on the instrument.